Id gene expression during development and molecular cloning of the human Id-1 gene.

Abstract

Id genes encode helix-loop-helix proteins that inhibit transcription by forming inactive heterodimers with basic helix-loop-helix (bHLH) proteins. bHLH proteins normally form either homodimers or heterodimers with other bHLH proteins and bind to a DNA sequence element activating transcription. Id-containing heterodimers are inactive because Id proteins lack the basic amino acid region necessary to form a DNA-binding domain. We have examined the relative levels of Id-1 and Id-2 mRNA during normal development and in malignant tissues. In the course of these experiments we cloned and sequenced the human Id-1 cDNA. Two related cDNA molecules encoding human Id-1 mRNAs were identified. Id-1a is a cDNA of 958 nucleotides and can encode a protein of 135 amino acids. Id-1b cDNA is 1145 nucleotides, can encode a protein of 149 amino acids, and appears to be a splice variant of Id-1a. The amino acid sequence of human Id-1 is greater than 90% homologous to that of mouse Id-1. The patterns of Id-1 and Id-2 expression during mouse development vary widely, and we detected Id-1 expression in human fetal and adult tissues from lung, liver, and brain. High Id-1 mRNA expression was found in many human tumor cell lines, including those isolated from nervous system tumors. We mapped Id-2 to human chromosome 2p25.

• Authors: W Zhu, J Dahmen, A Bulfone, M Rigolet, M C Hernandez, W L Kuo, L Puelles, J L Rubenstein, M A Israel
• Journal: Brain research. Molecular brain research (Brain Res Mol Brain Res) Vol. 30 Issue 2 Pg. 312-26 (Jun 1995) ISSN: 0169-328X [Print] Netherlands
• PMID: 7637581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Basic Helix-Loop-Helix Transcription Factors
• DNA-Binding Proteins
• ID1 protein, human
• Idb1 protein, mouse
• Inhibitor of Differentiation Protein 1
• Repressor Proteins
• Transcription Factors

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Basic Helix-Loop-Helix Transcription Factors
• Cells, Cultured
• Cloning, Molecular
• DNA-Binding Proteins (genetics)
• Gene Expression
• Helix-Loop-Helix Motifs (genetics)
• Humans
• In Situ Hybridization
• Inhibitor of Differentiation Protein 1
• Mice
• Molecular Sequence Data
• Repressor Proteins
• Transcription Factors (genetics)
• Transcription, Genetic
• Tumor Cells, Cultured