Synthesis and antitumor activity of various 6-demethylmitomycins and 6-demethyl-6-halomitomycins.

Abstract

A series of 6-demethylmitomycins and 6-demethyl-6-halomitomycins having various mitomycin skeletons were synthesized, taking into account the electronic effect toward the quinone moiety and the partition coefficients. Treatment of enones 15 and 16 with selenenamide or N-halosuccinimide-Et2NH afforded the 6-demethyl intermediates 17, 18, and 21-24 via the tandem Michael addition/retro-Mannich reaction sequence. Subsequent conversions into the mitomycin skeletons resulted in the formation of the desired derivatives 7a-c, 8a-c, 11a-c, and 12a,b. These mitomycin derivatives including 3a-c and 4a-c were evaluated for their anticellular activity against HeLa S3 cells and antitumor activity against Sarcoma 180 in mice. The anticellular activity of 1 and 3a-c depends on the substituent at the C-6 position and the order of increasing activity is H < CH3 < Br < Cl. A similar tendency was observed in their antitumor potency (ED50). The activities of 9 and 11a-c also follow a pattern similar to that of 1 and 3a-c. Compounds 4b,c, 8b,c, and 12b having both a halogen at the C-6 position and a methoxy group at the C-7 position did not show the activities because of the instability of the compounds. Interestingly, a correlation between the anticellular activity (IC50) and the partition coefficients (log kappa') determined by HPLC was observed within the compounds studied except the unstable compounds, while their antitumor activity (ED50 or T/C) did not correlate with the quinone reduction potential (E1/2). These results would indicate the importance of the C-6 substituents and the mitomycin skeletons for exhibiting both anticellular and antitumor activities.

• Authors: H Arai, T Ashizawa, K Gomi, M Kono, H Saito, M Kasai
• Journal: Journal of medicinal chemistry (J Med Chem) Vol. 38 Issue 16 Pg. 3025-33 (Aug 04 1995) ISSN: 0022-2623 [Print] United States
• PMID: 7636865 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Mitomycins

Topics

• Animals
• Antineoplastic Agents (chemical synthesis, pharmacology)
• Drug Screening Assays, Antitumor
• HeLa Cells
• Humans
• Mice
• Mitomycins (chemical synthesis, pharmacology)
• Sarcoma 180 (drug therapy)
• Structure-Activity Relationship