Inositol hexaphosphate inhibits growth and induces differentiation of PC-3 human prostate cancer cells.

Abstract

We investigated the effects of inositol hexaphosphate (InsP6) on growth inhibition and differentiation of human prostate cancer cells PC-3 in vitro. A significant dose- and time-dependent growth inhibition was observed as tested by the MTT-incorporation assay (P < 0.05 at 1 mM InsP6 after 24 h treatment, P < 0.01 at 0.1 mM after 3 days). DNA synthesis as determined by [3H]thymidine incorporation assay was also suppressed by InsP6 in a dose-dependent manner, occurring as early as 3 h after treatment and continuing up to 48 h (P < 0.01 at 1 mM InsP6). A 9- to 10-fold increase (P < 0.01) in expression of HLA class I molecule associated with tumor immunosurveillance and cell differentiation was induced by InsP6. The marker for prostatic cell differentiation, prostate acid phosphatase, was significantly (P < 0.05) increased after 48 h treatment at 0.5-5 mM InsP6. Since InsP6 strongly inhibits growth and induces differentiation in human prostate cancer cells in vitro, in vivo studies using a tumor xenograft model and a prostate carcinogenesis model are warranted to validate the efficacy of InsP6 in the treatment and prevention of prostate cancer.

• Authors: A M Shamsuddin, G Y Yang
• Journal: Carcinogenesis (Carcinogenesis) Vol. 16 Issue 8 Pg. 1975-9 (Aug 1995) ISSN: 0143-3334 [Print] England
• PMID: 7634429 (Publication Type: Journal Article)

Chemical References

• Histocompatibility Antigens Class I
• Phytic Acid
• DNA
• Acid Phosphatase

Topics

• Acid Phosphatase (analysis)
• Cell Differentiation (drug effects)
• Cell Division (drug effects)
• DNA (biosynthesis)
• Histocompatibility Antigens Class I (analysis)
• Humans
• Male
• Phytic Acid (pharmacology, therapeutic use)
• Prostatic Neoplasms (drug therapy, pathology, prevention & control)
• Tumor Cells, Cultured