Plasma 8-epi-PGF2 alpha levels are elevated in individuals with non-insulin dependent diabetes mellitus.

Abstract

This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyzed by GC-MS/NICI as PFB-ester/TMS-ether derivative. We found that the average concentration of esterified 8-epi-PGF2 alpha among NIDDM subjects (0.93 +/- 0.07 nM, n = 39) was higher (P < 0.0001, Mann-Whitney test) than in healthy individuals (0.28 +/- 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.

• Authors: N K Gopaul, E E Anggård, A I Mallet, D J Betteridge, S P Wolff, J Nourooz-Zadeh
• Journal: FEBS letters (FEBS Lett) Vol. 368 Issue 2 Pg. 225-9 (Jul 17 1995) ISSN: 0014-5793 [Print] England
• PMID: 7628610 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 8-epi-prostaglandin F2alpha
• Dinoprost
• Aspirin
• Indomethacin

Topics

• Adult
• Aspirin (pharmacology)
• Chromatography, Gas (methods)
• Diabetes Mellitus, Type 2 (blood)
• Dinoprost (analogs & derivatives, biosynthesis, blood)
• Esterification
• Humans
• Indomethacin (pharmacology)
• Mass Spectrometry (methods)
• Models, Chemical
• Oxidative Stress