Abstract | BACKGROUND: STUDY DESIGN: Fecal peritonitis was induced by intraperitoneal injection of sterile feces contaminated with 10(8) (experiment 1) or 10(4) (experiment 2) colony forming units (cfu) E. coli and 10(4) cfu Bacteroides fragilis. Male Wistar rats were randomly assigned to receive either methyl hydroxy propyl cellulose (MHPC) gel alone (M) or 0.5 mg/mL rtPA dissolved in MHPC gel (M-tPA). Three hours after inoculation, one-half of the rats in each of these groups received 6 mg/kg gentamycin sulfate (G) intramuscularly (group M-G and M-tPA-G). At one, three, six, 12, and 24 hours after inoculation, blood cultures were taken. At five days after inoculation, intra-abdominal abscess formation was assessed and abscesses were cultured (experiment 2). RESULTS: All rats in groups M and M-tPA in experiment 1 developed bacteremia and died within 24 hours. Bacteremia occurred significantly earlier in group M-tPA compared with group M (p < 0.05). Gentamycin significantly reduced the number of rats with bacteremia, the bacteria concentration in the blood, and mortality rates. Although in experiment 2 none of the rats developed bacteremia, gentamycin prevented mortality associated with the use of rtPA. The number of abscesses in groups M-tPA and M-tPA-G was significantly lower than in those in groups M and M-G (p < 0.01). Gentamycin did not influence the number of abscesses. CONCLUSIONS:
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Authors | H van Goor, J S de Graaf, K Kooi, R P Bleichrodt |
Journal | Journal of the American College of Surgeons
(J Am Coll Surg)
Vol. 181
Issue 1
Pg. 38-42
(Jul 1995)
ISSN: 1072-7515 [Print] United States |
PMID | 7599769
(Publication Type: Journal Article)
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Chemical References |
- Gentamicins
- Tissue Plasminogen Activator
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Topics |
- Abdominal Abscess
(prevention & control)
- Animals
- Bacteremia
(etiology, microbiology, prevention & control)
- Escherichia coli Infections
(prevention & control)
- Gentamicins
(therapeutic use)
- Male
- Peritonitis
(drug therapy)
- Rats
- Rats, Wistar
- Tissue Plasminogen Activator
(therapeutic use)
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