Studies on T-cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach.

Abstract

Based upon Blalock's complementary recognition approach, a complementary or antisense peptide (CP) was designed to the experimental autoimmune encephalomyelitis (EAE) epitope peptide, rat myelin basic protein (MBP) peptide 72-82. This peptide (EAE CP) was shown to have some sequence similarities to T-cell receptors (TCR) and MHC II molecules in a sequence homology search. Solid-phase binding assays demonstrated specific and high affinity binding (3 and 4 microM) between the EAE CP and the rat and guinea pig EAE epitope peptides (Rt72-82 and Gp69-82), respectively. This EAE CP was also found to be immunogenic in rats in an ear swelling test for delayed type hypersensitivity (DTH) reactions and an ELISA for antibody responses. However, a rabbit antibody generated to EAE CP was shown to be unable to stain the V beta 8+ EAE susceptible T-cells in immunofluorescence analyses. This EAE CP was also used in attempts to down-regulate EAE and the results showed that prior immunization with EAE CP in complete Freund's adjuvant could not prevent the Lewis rats from developing EAE. Although the data on sense-antisense peptide interaction were positive and the EAE CP was immunogenic, the inability of EAE CP to regulate EAE indicates that the CP approach may not be generally applicable.

• Authors: C J Xian, R D Simmons, D O Willenborg, A A Vandenbark, G A Hashim, P R Carnegie
• Journal: Journal of neuroscience research (J Neurosci Res) Vol. 41 Issue 5 Pg. 620-7 (Aug 01 1995) ISSN: 0360-4012 [Print] United States
• PMID: 7563242 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antisense Elements (Genetics)
• DNA, Complementary
• Epitopes
• Histocompatibility Antigens Class II
• Immunoglobulin G
• Myelin Basic Protein
• Peptide Fragments
• Receptors, Antigen, T-Cell

Topics

• Amino Acid Sequence
• Animals
• Antisense Elements (Genetics) (immunology, metabolism)
• Base Sequence
• DNA, Complementary (genetics)
• Encephalomyelitis, Autoimmune, Experimental (immunology, physiopathology, prevention & control)
• Epitopes
• Genetic Techniques
• Guinea Pigs
• Histocompatibility Antigens Class II (genetics)
• Immunoglobulin G (immunology)
• Molecular Mimicry
• Molecular Sequence Data
• Myelin Basic Protein (genetics, metabolism)
• Peptide Fragments (genetics, metabolism)
• Rabbits
• Rats
• Receptors, Antigen, T-Cell (genetics, physiology)
• T-Lymphocytes (immunology)