Methylation of cottontail rabbit papillomavirus DNA and tissue-specific expression in transgenic rabbits.

Abstract

Transgenic rabbits carrying multiple copies of CRPV genomic DNA were described previously (Peng et al. (1993) J. Virol. 67, 1698-1701). CRPV DNA was detectable in all tissues of the transgenic rabbits, however, the transcripts of CRPV genes only were found in skin and skin tumors. Tumor development was also restricted to skin. To study the mechanism involving tissue-specific expression of CRPV genes in rabbits, cellular DNAs, isolated from different normal tissues and skin tumors, were digested with the two isoschizomeric restriction endonucleases MspI (methylation resistant) and HpaII (methylation sensitive), respectively, and analyzed by Southern blot. CRPV DNA, especially its upstream regulatory region (URR), was extensively methylated in all normal tissues, but methylation was remarkably reduced in skin tumors. These data suggest that extensive methylation of CRPV genome, especially in the URR, might be a factor in controlling its tissue-specific expression.

• Authors: X Peng, C M Lang, J W Kreider
• Journal: Virus research (Virus Res) Vol. 35 Issue 1 Pg. 101-8 (Jan 1995) ISSN: 0168-1702 [Print] Netherlands
• PMID: 7538718 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Recombinant
• DNA, Viral
• 5-Methylcytosine
• Cytosine
• DNA (Cytosine-5-)-Methyltransferases
• Deoxyribonuclease HpaII
• Deoxyribonucleases, Type II Site-Specific

Topics

• 5-Methylcytosine
• Animals
• Animals, Genetically Modified
• Cottontail rabbit papillomavirus (genetics)
• Cytosine (analogs & derivatives, analysis)
• DNA (Cytosine-5-)-Methyltransferases (metabolism)
• DNA, Recombinant (pharmacokinetics)
• DNA, Viral (chemistry, genetics)
• Deoxyribonuclease HpaII
• Deoxyribonucleases, Type II Site-Specific (metabolism)
• Gene Expression Regulation, Neoplastic
• Gene Expression Regulation, Viral
• Methylation
• Microinjections
• Organ Specificity
• Papilloma (veterinary, virology)
• Papillomavirus Infections (veterinary)
• Rabbits (virology)
• Regulatory Sequences, Nucleic Acid
• Skin (metabolism, virology)
• Skin Neoplasms (veterinary, virology)
• Tissue Distribution
• Tumor Virus Infections (veterinary)