Effect of PAF antagonists on cerulein-induced pancreatitis.

Abstract

The present study was undertaken to investigate the involvement of PAF in acute pancreatitis induced by cerulein in rats. Cerulein (two doses of 20 micrograms/rat, the first s.c. and the second i.v., 1 h apart) induced a significant increase in vascular permeability in the pancreas, evaluated by the Evans blue (EB) extravasation method. Plasma amylase levels were also significantly increased in this group. The PAF antagonists, BN-52021 (5 mg/kg) and WEB-2170 (1 and 10 mg/kg), both significantly reduced the extravasation of EB in the pancrease induced by i.v. injection of PAF (1 microgram/kg). At these concentrations, BN-52021 was effective at inhibiting cerulein-induced pancreatitis (60-70% of inhibition) whereas WEB-2170 had no significant effect. Although the inhibition induced by BN-52021 suggests the involvement of PAF in cerulein-pancreatitis, the lack of effect of WEB-2170 reported here does not allow a definite conclusion. Further studies are needed to elucidate the differential effect of the PAF antagonists.

• Authors: S Jancar, E E Abdo, S N Sampietre, F H Kwasniewski, A M Coelho, A Bonizzia, M C Machado
• Journal: Journal of lipid mediators and cell signalling (J Lipid Mediat Cell Signal) Vol. 11 Issue 1 Pg. 41-9 (Jan 1995) ISSN: 0929-7855 [Print] Netherlands
• PMID: 7537159 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Azepines
• Diterpenes
• Ginkgolides
• Lactones
• Platelet Activating Factor
• Triazoles
• Evans Blue
• Ceruletide
• bepafant
• ginkgolide B
• Amylases

Topics

• Acute Disease
• Amylases (blood)
• Animals
• Azepines (pharmacology)
• Capillary Permeability (drug effects)
• Ceruletide (toxicity)
• Diterpenes
• Evans Blue (pharmacokinetics)
• Ginkgolides
• Lactones (pharmacology)
• Male
• Pancreas (blood supply)
• Pancreatitis (chemically induced, drug therapy, enzymology)
• Platelet Activating Factor (antagonists & inhibitors)
• Rats
• Rats, Wistar
• Triazoles (pharmacology)