In vitro bioassay with enhanced sensitivity for human granulocyte colony-stimulating factor.

Abstract

A method for the determination of human granulocyte colony-stimulating factor (hG-CSF) activity, based on stimulation of cellular proliferation, was developed using a subclone of the murine myeloid leukemia cell line NFS-60, with an improved sensitivity for hG-CSF, as indicator. The optimal range for quantitative analysis of hG-CSF was about 4-60 pg ml-1. The stimulatory effect was measured by a colorimetric microassay: the optical density of formazan, which is produced by viable cells from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), was obtained by reading plates in a multi-channel photometer. The assay was designed as a five-dose parallel line test, employing three or four doses for potency determinations, which fulfil pharmacopoeial requirements for assay validity. Inter-assay relative standard deviation (RSD) varied between 5.2 and 12.0%. Most assay experiments revealed potencies within limits of error of 90-110% and the mean index of precision value was 0.057. The recently developed yeast cell-derived International Standard (88/502) served as a reference for activity of rhG-CSF. Specificity of the assay was demonstrated by absence of response upon exposure to a panel of biomolecules, including recombinant human interleukin-3, and by the suppression of growth stimulation in the presence of neutralizing anti hG-CSF antibodies. Potency readings of unglycosylated rhG-CSF were dependent on pH of assay medium with higher relative activities observed at pH 6.6 than at 7.4. Moreover, SDS-PAGE analysis of the carbohydrate-deficient preparation, following incubation at physiological pH, revealed several high molecular weight rhG-CSF bands and decreased monomeric form. The method described was found suitable for potency assessments of pharmaceutical formulations of hG-CSF.

• Authors: U Hammerling, R Kroon, L Sjödin
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 13 Issue 1 Pg. 9-20 (Jan 1995) ISSN: 0731-7085 [Print] England
• PMID: 7536474 (Publication Type: Journal Article)

Chemical References

• Formazans
• Recombinant Proteins
• Tetrazolium Salts
• Granulocyte Colony-Stimulating Factor
• MTT formazan
• Sodium Dodecyl Sulfate

Topics

• Animals
• Cell Division (drug effects)
• Electrophoresis, Polyacrylamide Gel
• Evaluation Studies as Topic
• Formazans
• Granulocyte Colony-Stimulating Factor (metabolism, pharmacology)
• Heating
• Humans
• Leukemia, Myelomonocytic, Acute (pathology)
• Mice
• Photometry
• Recombinant Proteins (analysis, pharmacology)
• Sensitivity and Specificity
• Sodium Dodecyl Sulfate
• Tetrazolium Salts
• Tumor Cells, Cultured (drug effects)