Characterization of a secreted form of measles virus haemagglutinin expressed from a vaccinia virus recombinant.

Abstract	The measles virus (MV) haemagglutinin (HA) is a class 2 glycoprotein by means of which the virus particle attaches to the host cell receptor. We have previously expressed this glycoprotein as a vaccinia recombinant virus and have shown that the HA glycoprotein synthesized is indistinguishable from that coded by MV. In the present study, we report that in RK13 cells a soluble form (sHA) of the HA is secreted into the medium. We show by SDS-PAGE and sucrose density gradient centrifugation that the sHA is a dimer and is smaller than the cell-associated form. Using a variety of inhibitors the production of sHA was shown to be a late event, probably occurring at the membrane; only fully glycosylated molecules were found in sHA. Finally, we demonstrate that sHA retains its antigenicity with conformation-dependent MAbs and its receptor recognition function. We conclude that sHA is a valuable tool for use in studies of the structure and function of the MV HA glycoprotein.
Authors	E Malvoisin, F Wild
Journal	The Journal of general virology (J Gen Virol) Vol. 75 ( Pt 12) Pg. 3603-9 (Dec 1994) ISSN: 0022-1317 [Print] England
PMID	7527839 (Publication Type: Journal Article)
Chemical References	Antibodies, Monoclonal Antibodies, Viral Antigens, CD Antigens, Viral Epitopes Hemagglutinins, Viral Membrane Cofactor Protein Membrane Glycoproteins Protein Synthesis Inhibitors Receptors, Virus Recombinant Proteins hemagglutinin protein G, measles virus Hexosaminidases
Topics	Antibodies, Monoclonal Antibodies, Viral Antigens, CD (metabolism) Antigens, Viral (metabolism) Biological Transport Cell Line Cloning, Molecular Epitopes (analysis) Glycosylation Hemagglutinins, Viral (biosynthesis, chemistry, genetics, metabolism) Hexosaminidases Measles virus (chemistry, metabolism) Membrane Cofactor Protein Membrane Glycoproteins (metabolism) Molecular Weight Protein Conformation Protein Synthesis Inhibitors Receptors, Virus (metabolism) Recombinant Proteins (biosynthesis, chemistry, metabolism) Solubility Vaccinia virus (genetics)

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