Identification of a novel neutralization epitope on envelope gp46 antigen of human T-cell-leukemia virus-type-II (HTLV-II).

Abstract

Having observed that multiple neutralization epitopes of human T-cell-leukemia-virus-type-I (HTLV-I) envelope gp46 clustered in a region between amino acids 187 and 199, we speculated that a region of HTLV-II gp46 corresponding to the HTLV-I-neutralization region may contain HTLV-II-specific neutralization epitopes. To test this, we immunized a NZW rabbit and BALB/c mice with a synthetic peptide containing the HTLV-II gp46 amino acids 182 to 199 (pep182-199) conjugated to OVA. The serum from the rabbit reacted to the HTLV-II gp46 and neutralized HTLV-II-mediated syncytium formation. One monoclonal antibody (MAb), M2E186N1, generated from the BALB/c mice, stained specifically the surface of HTLV-II-positive cells and reacted with HTLV-II gp46 by Western blot. This MAb was of the IgA isotype and inhibited HTLV-II- but not HTLV-I-mediated syncytium formation at a final antibody concentration of 200 micrograms/ml; it also neutralized an HTLV-II-VSV pseudotype virus specifically. Epitope mapping by ELISA using overlapping synthetic peptides indicated that the neutralization epitope recognized by the M2E186N1 MAb was located between HTLV-II gp46 amino acids 186 and 192, corresponding to the sequence Leu-Gln-His-Val-Ile-Leu-Gln-Pro. These new observations will be helpful in developing vaccines against HTLV-II infection.

• Authors: Y Tanaka, R Tanaka, H Hoshino
• Journal: International journal of cancer (Int J Cancer) Vol. 59 Issue 5 Pg. 655-60 (Dec 01 1994) ISSN: 0020-7136 [Print] United States
• PMID: 7525496 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Epitopes
• Gene Products, env
• HTLV-II Antigens
• Immunoglobulin A
• Retroviridae Proteins, Oncogenic
• env Gene Products, Human Immunodeficiency Virus
• gp46 protein, Human immunodeficiency virus 2

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• Epitopes (analysis)
• Gene Products, env (chemistry, immunology)
• HTLV-II Antigens (analysis)
• Human T-lymphotropic virus 2 (immunology)
• Humans
• Immunoglobulin A
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Rabbits
• Retroviridae Proteins, Oncogenic (chemistry, immunology)
• env Gene Products, Human Immunodeficiency Virus