Identification of the disease-related T cell epitope of ovalbumin and epitope-targeted T cell inactivation in egg allergy.
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| Abstract |
An ovalbumin (OVA)-specific T cell line (TCL) was established from a patient with hen egg allergy. The TCL was CD4+, expressed alpha beta T cell receptor, and recognized OVA presented by HLA-DR10. Based on the response of the TCL to synthetic OVA peptides, it was found that the TCL recognized OVA 323-339, which is a major T cell epitope presented by murine I-Ad. The TCL secreted high levels of IL-5, but undetectable amounts of IL-2, interferon-gamma, and IL-4 when stimulated with OVA or the OVA 323-339 peptide. Since IL-5 is an important growth and chemotactic factor for eosinophils, it is possible that these OVA 323-339-specific T cells can contribute to human egg allergy. To our knowledge, this is the first demonstration of food allergen-specific TCL establishment and identification of a T cell epitope possibly related to the allergic reaction to food antigens. An analog peptide of the OVA 323-339 which is known to strongly bind to I-Ad partially inhibited the response of the TCL to OVA 323-339 presented by HLA-DR10, raising the possibility of peptide-based immunotherapy of food allergy.
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| Authors | N Shimojo, T Katsuki, J E Coligan, Y Nishimura, T Sasazuki, H Tsunoo, T Sakamaki, Y Kohno, H Niimi |
| Journal | International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 105 Issue 2 Pg. 155-61 (Oct 1994) ISSN: 1018-2438 [Print] Switzerland |
| PMID | 7522686 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't) |
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