Abstract |
Interleukin-2 (IL-2)-activated killer cells, also referred to as lymphokine-activated killer (LAK) cells, are stimulated by tumor cells to express cytotoxic activity and to also secrete cytokines such as interferon gamma (IFN gamma) and tumor necrosis factor alpha ( TNF alpha). We previously reported that secretion of cytokines by IL-2-activated T cells (LAK-T cells) is dependent on the initial cross-linking of the T cell receptor (TCR)-CD3-molecular complex, but the cross-linking of accessory molecules, such as LFA-1, CD2, CD44 and CD45, on LAK-T cells can enhance this cytokine production. We have developed an approach involving interspecific gene transfer to define further the contributions of LFA-1 and CD2 to the activation of LAK-T cells. The genes for huICAM-1 (a ligand for LFA-1) and huLFA-3 (a ligand for CD2) were transfected singly and in combination into a null mouse melanoma background, and clonal populations of cells that stably express ICAM-1 and/or LFA-3 were derived. Expression of the introduced ICAM-1 and/or LFA-3 by transfected cells enhanced their ability to bind LAK-T cells; the LFA-1/ICAM-1-mediated binding was not further enhanced by activation with phorbol 12-myristate 13-acetate. ICAM-1- and/or LFA-3-transfected cells, in the presence of immobilized anti-CD3, exhibited a greater ability to stimulate IFN gamma secretion by LAK-T cells compared to the untransfected parental lines. This experimental system, which allows ICAM-1/LFA-1 and CD2/LFA-3 interactions to occur on the LAK-T cell at a site distal from the anti-CD3 signal, extends our understanding of LAK-T cell activation by establishing that both LFA-1/ICAM-1 and CD2/LFA-3 can mediate co-stimulation via adhesion and signaling events.
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Authors | A S Chong, X L Jiang, P Scuderi, M Lamas, L H Graf Jr |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
Vol. 39
Issue 2
Pg. 127-34
(Aug 1994)
ISSN: 0340-7004 [Print] Germany |
PMID | 7519126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, CD
- Antigens, Differentiation, T-Lymphocyte
- CD2 Antigens
- CD3 Complex
- CD58 Antigens
- Cell Adhesion Molecules
- Interleukin-2
- Membrane Glycoproteins
- Receptors, Immunologic
- Intercellular Adhesion Molecule-1
- Interferon-gamma
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Topics |
- Antibodies, Monoclonal
(pharmacology)
- Antigens, CD
(genetics, physiology)
- Antigens, Differentiation, T-Lymphocyte
(genetics, physiology)
- CD2 Antigens
- CD3 Complex
(immunology)
- CD58 Antigens
- Cell Adhesion
(physiology)
- Cell Adhesion Molecules
(genetics, physiology)
- Humans
- Intercellular Adhesion Molecule-1
- Interferon-gamma
(biosynthesis)
- Interleukin-2
(pharmacology)
- Killer Cells, Lymphokine-Activated
(cytology, drug effects, metabolism)
- Lymphocyte Activation
(drug effects)
- Membrane Glycoproteins
(genetics, physiology)
- Phenotype
- Receptors, Immunologic
(genetics, physiology)
- Stimulation, Chemical
- T-Lymphocytes
(cytology, drug effects, metabolism)
- Transfection
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