Immunization with the paraneoplastic encephalomyelitis antigen HuD does not cause neurologic disease in mice.

Abstract

Paraneoplastic encephalomyelitis/paraneoplastic sensory neuronopathy (PEM/PSN) associated with small cell lung cancer is characterized by high serum and CSF titers of anti-neuronal (anti-Hu) antibodies and by intrathecal synthesis of anti-Hu IgG. A pathologic role for the anti-Hu antibodies in PEM/PSN is further suggested by reported intraneuronal accumulation of the antibodies in the nervous system of PEM/PSN patients at autopsy. We immunized SJL/J mice, Lewis rats, and Hartley guinea pigs with purified recombinant HuD fusion protein. In spite of high-titer anti-HuD antibodies, neurologic and pathologic examination of the animals was normal. Apparent uptake of purified IgG by neurons in the brain proved to be artifactual.

• Authors: P A Sillevis Smitt, G T Manley, J B Posner
• Journal: Neurology (Neurology) Vol. 45 Issue 10 Pg. 1873-8 (Oct 1995) ISSN: 0028-3878 [Print] United States
• PMID: 7477985 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• ELAV Proteins
• ELAV-Like Protein 4
• ELAVL4 protein, human
• Elavl4 protein, mouse
• Nerve Tissue Proteins
• RNA-Binding Proteins

Topics

• Animals
• Antibodies (administration & dosage)
• Antibody Formation
• Base Sequence
• Brain (pathology)
• ELAV Proteins
• ELAV-Like Protein 4
• Encephalomyelitis (immunology, pathology)
• Guinea Pigs
• Humans
• Immunization
• Immunohistochemistry
• Mice
• Molecular Sequence Data
• Nerve Tissue Proteins
• Paraneoplastic Syndromes (immunology, pathology)
• RNA-Binding Proteins (administration & dosage, immunology)
• Rats