Chemical studies of tissue polypeptide antigen (TPA). II. Partial amino acid sequences of cyanogen bromide fragments of TPA subunit B1.

Abstract

A subunit (designated B1) of the tumor associated peptide "Tissue Polypeptide Antigen" (TPA) has been cleaved by cyanogen bromide. Some of the fragments contained TPA-activity (as measured by a standardized hemagglutination inhibition method). The fragments have been separated, and partial amino acid sequences have been determined for some of the fragments. A method for purification of the most active fragment (designated BrCN:C) has been worked out from cleavage products obtained by cleavage with cyanogen bromide of "Washed Tissue Powder". By this method fragment BrCN:C has been isolated from a pool of placentae and a pool or carcinoma tumors, and the amino acid sequences of more than 50 amino acids from the N-terminus have been determined. Fragment BrCN:C has been demonstrated from three different sources, which indicates the presence of a common part in subunit B1 independent of source.

• Authors: P Redelius, B Lüning, B Björklund
• Journal: Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry (Acta Chem Scand B) Vol. 34 Issue 4 Pg. 265-73 ( 1980) ISSN: 0302-4369 [Print] Denmark
• PMID: 7435086 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Antigens, Neoplasm
• Peptide Fragments
• Peptides
• Tissue Polypeptide Antigen
• Cyanogen Bromide

Topics

• Amino Acid Sequence
• Amino Acids (analysis)
• Antigens, Neoplasm
• Chemical Phenomena
• Chemistry
• Chromatography, High Pressure Liquid
• Cyanogen Bromide
• Humans
• Peptide Fragments (analysis, isolation & purification)
• Peptides
• Tissue Polypeptide Antigen