Activation of human neutrophil metabolism by streptolysin O.

Abstract

The effect of streptolysin O on the metabolic activity of human neutrophils was examined. Streptolysin O, within the range of 3 to 75 hemolytic units, stimulated neutrophils to emit light. This chemiluminescence was the result of metabolic activation, as confirmed by oxygen uptake studies and the suppression of chemiluminescence by the metabolic inhibitor, 2-deoxyglucose. Free cholesterol, which prevents streptolysin O from binding to membrane cholesterol, blocked the neutrophil chemiluminescent response to streptolysin O. Extracellular calcium ions were necessary for the streptolysin O-stimulated chemiluminescent response of neutrophils. Some cell viability was lost, as measured by trypan blue uptake and neutrophil lysis within the range of streptolysin O concentration that caused metabolic stimulation. These observations suggest a possible role for streptolysin O as a pathogenic factor in streptococcal infections.

• Authors: B R Andersen, J L Duncan
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 141 Issue 5 Pg. 680-5 (May 1980) ISSN: 0022-1899 [Print] United States
• PMID: 7373090 (Publication Type: Journal Article)

Chemical References

• Dinitrophenols
• Streptolysins
• Deoxyglucose
• Magnesium
• Oxygen
• Calcium

Topics

• Calcium (metabolism)
• Cell Survival
• Deoxyglucose (pharmacology)
• Dinitrophenols (pharmacology)
• Humans
• Luminescent Measurements
• Magnesium (metabolism)
• Neutrophils (metabolism)
• Oxygen (metabolism)
• Streptolysins (pharmacology)