The ultraviolet-induced
erythema reaction was investigated histologically and biochemically in four subjects, utilizing suction
blister aspirates, analyzed for
histamine and
prostaglandin E2 (
PGE2), and
Epon-embedded 1-mu skin biopsy sections from control skin and from irradiated skin at intervals for 72 hours after exposure to a Hanovia lamp. Major histologic alterations in the epidermis included dyskeratotic and vacuolated keratinocytes (
sunburn cells), and disappearance of Langerhans cells. In the dermis the major changes were vascular, involving both the superficial and deep venular plexuses. Endothelial cell enlargement was first apparent within 30 minutes of irradiation, peaked at 24 hours, and persisted throughout the 72-hour study period. Mast cell degranulation and associated perivenular
edema were first apparent at 1 hour and striking at the onset of
erythema, 3 to 4 hours postirradiation;
edema was absent and mast cells were again normal in number and granule content at 24 hours.
Histamine levels rose approximately fourfold above control values immediately after the onset of
erythema and returned to baseline within 24 hours.
PGE2 levels were statistically elevated even before the onset of
erythema and reached approximately 150% of the control value at 24 hours. These data provide the first evidence that
histamine may mediate the early phase of the human
sunburn reaction and increase our understanding of its complex histologic and biochemical sequelae.