Evidence for a defective mitochondrial membrane in 1,2-dimethylhydrazine-induced colon adenocarcinoma in rat: enhanced lipid peroxidation potential in vitro.

Abstract

Enhanced lipid peroxidation potential was measured in Holtzman rat colon tumors induced by chronic subcutaneous injection of 1,2-dimethyl-hydrazine as compared with normal colonic tissue. The peroxidation potentials were determined in the mitochondrial cellular components by measuring the ferrous-ascorbate induced formation of malondialdehyde. The tumor mitochondria were found to peroxidize at a rate 8-10-fold higher than the comparable normal tissue components. In addition, we found that the mitochondria from the cancer cells exhibited reduced NADH-cytochrome c reductase activity. These observations suggest an involvement of non-enzymatic free radical flux in DMH-induced carcinogenesis, which may be the result of structurally altered mitochondrial membranes.

• Authors: R S Rana, R H Stevens, L Oberley, D P Loven, J M Graves, D A Cole, E S Meek
• Journal: Cancer letters (Cancer Lett) Vol. 9 Issue 3 Pg. 237-44 (May 1980) ISSN: 0304-3835 [Print] Ireland
• PMID: 7226156 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Dimethylhydrazines
• Lipid Peroxides

Topics

• Adenocarcinoma (chemically induced, metabolism)
• Animals
• Cell Membrane (pathology)
• Colonic Neoplasms (chemically induced, metabolism)
• Dimethylhydrazines
• Lipid Peroxides (metabolism)
• Male
• Mitochondria (metabolism)
• Rats