Inhibitory effects of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) in the response of isolated hepatocytes to phalloidin.

Abstract

4,4'-Diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) inhibits the typical development of protrusions, regularly seen after treatment of isolated hepatocytes with phalloidin. The degree of inhibition depends on the time of preincubation and on the concentration of DIDS, but not on the concentration of phalloidin. DIDS is more effective than H2DIDS. The inhibition by both compounds is irreversible. The binding capacity of hepatocytes for H2DIDS is much higher than that of the phalloidin-insensitive hepatoma cells. Gel electrophoresis of lysates from cells, pretreated with 3H2DIDS demonstrates that actin binds very little of the inhibitor. Our results suggest that a protein structure on the surface of hepatocytes, needed for the response to phalloidin, is influenced by DIDS or H2DIDS.

• Authors: E Petzinger, E Grundmann, L B Veil, M Frimmer, H Fasold
• Journal: Naunyn-Schmiedeberg's archives of pharmacology (Naunyn Schmiedebergs Arch Pharmacol) Vol. 304 Issue 3 Pg. 303-7 (Oct 1978) ISSN: 0028-1298 [Print] Germany
• PMID: 714189 (Publication Type: Journal Article)

Chemical References

• Oligopeptides
• Proteins
• Stilbenes
• Phalloidine

Topics

• Animals
• Electrophoresis, Polyacrylamide Gel
• In Vitro Techniques
• Liver (cytology, drug effects)
• Oligopeptides (antagonists & inhibitors)
• Phalloidine (antagonists & inhibitors, pharmacology)
• Proteins (analysis)
• Rats
• Stilbenes (pharmacology)
• Time Factors