Abstract |
Suppressor cell activity in intestinal lymphocytes was investigated by a coculture assay with autologous peripheral blood lymphocytes. Greater than 45% suppression was found in 7 out of 10 patients without Crohn's disease. Reduced suppressor cell activity was found in intestinal lymphocytes isolated from 7 out of 8 patients with Crohn's disease. Intestinal lymphocytes from patients with Crohn's disease demonstrated a significantly greater response to phytohemagglutinin-P than lymphocytes isolated from non-Crohn's patients. However, this difference could not be explained by alterations in proportions of T lymphocytes as intestinal T lymphocytes from non- Crohn's disease patients (54%) were not significantly different from those found in patients with Crohn's disease (57%). Indomethacin, a prostaglandin synthetase inhibitor, decreased the proliferative response of intestinal lymphocytes to phytohemagglutinin-P whereas the response of peripheral blood lymphocytes was enhanced, thus providing further evidence that intestinal lymphocytes may represent a functionally distinct population from circulating lymphocytes. Cimetidine, a H2-receptor antagonist, had no effect on the response of intestinal lymphocytes to phytohemagglutinin-P. These results provide evidence to support the concept that disturbed immunoregulation at the mucosal level is found in Crohn's disease.
|
Authors | R L Goodacre, J Bienenstock |
Journal | Gastroenterology
(Gastroenterology)
Vol. 82
Issue 4
Pg. 653-8
(Apr 1982)
ISSN: 0016-5085 [Print] United States |
PMID | 7060886
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Mitomycins
- Phytohemagglutinins
- Cimetidine
- Indomethacin
|
Topics |
- Cimetidine
(pharmacology)
- Crohn Disease
(immunology)
- Humans
- Indomethacin
(pharmacology)
- Intestinal Mucosa
(cytology)
- Lymphocyte Activation
(drug effects)
- Lymphocytes
(drug effects, immunology)
- Mitomycins
(pharmacology)
- Phytohemagglutinins
(pharmacology)
- Rosette Formation
|