Potential identification of chemical carcinogens in a viral transformation system.

Abstract

Chemical carcinogens from several diverse chemical classes i.e.; aromatic amines, polycyclic hydrocarbons, nitrosamines, hormonal derivatives, metals and direct alkylating agents cause a 6.2-60.5-fold increase in the frequency of murine sarcoma virus (MSV)-induced transformation in a normal rat kidney (NRK) cell system. Exogenous metabolic activation with a rat liver S-9 homogenate is required for expression of this activity by procarcinogens. Non-carcinogenic analogs of these compounds fail to cause significant increases in the transformation frequency either with or without prior metabolic activation. Iododeoxyuridine, a mutagen also does not cause enhancement of transformation. This system may serve as the basis for a rapid and quantifiable means of identifying chemical carcinogens while introducing a new model for the understanding of the interactions between oncornaviruses and chemical carcinogens.

• Authors: W W Wilson, N Khoobyarian
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 38 Issue 2 Pg. 253-9 (Jan 1982) ISSN: 0009-2797 [Print] Ireland
• PMID: 7055852 (Publication Type: Journal Article)

Chemical References

• Carcinogens

Topics

• Animals
• Carcinogens (pharmacology)
• Cell Line
• Cell Transformation, Viral (drug effects)
• Kidney (drug effects, metabolism)
• Mice
• Moloney murine leukemia virus
• Rats