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Modified granulocyte test for determination of mannosidosis genotype of cattle.

Abstract
Recovery of alpha-mannosidase, beta-N-acetylglucosaminidase and arylsulphatase activity from granulocytes is increased by inclusion of 154 mM sodium chloride, in place of 1 mM zinc chloride, in aqueous Triton X-100 used to extract the cells. Calculation of the ratio of alpha-mannosidase to beta-N-acetylglucosaminidase and to arylsulphatase permits diagnosis of the mannosidosis genotype without recourse to examination of smears of the granulocyte preparations.
AuthorsP J Healy
JournalResearch in veterinary science (Res Vet Sci) Vol. 30 Issue 3 Pg. 281-3 (May 1981) ISSN: 0034-5288 [Print] England
PMID7020017 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chlorides
  • Zinc Compounds
  • Sodium Chloride
  • zinc chloride
  • Arylsulfatases
  • alpha-Glucosidases
  • Acetylglucosaminidase
  • Zinc
Topics
  • Acetylglucosaminidase (blood)
  • Animals
  • Arylsulfatases (blood)
  • Carbohydrate Metabolism, Inborn Errors (diagnosis, veterinary)
  • Cattle
  • Cattle Diseases (diagnosis)
  • Chlorides
  • Clinical Enzyme Tests (veterinary)
  • Granulocytes (enzymology)
  • Sodium Chloride (pharmacology)
  • Zinc (pharmacology)
  • Zinc Compounds
  • alpha-Glucosidases (blood)

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