Abstract |
A simple, sensitive, micromethod was developed to determine levels of ribavirin, or its active metabolic products, in human serum or urine. The procedure utilized the inhibition of measles virus cytopathic effect in BS-C-1 cells. Based upon maximum dilutions of human serum or urine containing ribavirin which inhibited the measles virus, the bioassay detected ribavirin in concentrations as low as 0.006 microgram/ml in serum or 0.03 microgram/ml in urine. Herpesvirus 1, parainfluenza virus 3 and reovirus 1 were also tested for sensitivity to ribavirin. Minimum inhibitory concentrations of ribavirin in serum or urine against these other viruses were no lower 0.32 microgram/ml.
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Authors | D F Smee, R W Sidwell, B B Barnett, R S Spendlove |
Journal | Chemotherapy
(Chemotherapy)
Vol. 27
Issue 1
Pg. 1-11
( 1981)
ISSN: 0009-3157 [Print] Switzerland |
PMID | 7011693
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ribonucleosides
- Ribavirin
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Topics |
- Biological Assay
(methods)
- Cytopathogenic Effect, Viral
(drug effects)
- Fluorescent Antibody Technique
- Humans
- Microbial Sensitivity Tests
- Ribavirin
(analysis)
- Ribonucleosides
(analysis)
- Viruses
(drug effects)
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