125I-labeled cell surface as a marker in preparation of microsome fraction by gel filtration.

Abstract

The microsomal fraction was isolated from homogenate of 125I-labeled leukemia L 1210 ascites cells by filtration of postmitochondrial supernatant through a Sepharose 4 B column. It was found that the particles are labeled with iodine and show 5'-nucleotidase activity suggesting the presence of cell membranes in the fraction. The soluble proteins fraction were retarded on the column showed lactate dehydrogenase activity, and low activity of soluble beta-D-glucuronidase.

• Authors: B Miks, J Kawiak, G Hoser
• Journal: Folia histochemica et cytochemica (Folia Histochem Cytochem (Krakow)) Vol. 16 Issue 3 Pg. 187-92 ( 1978) ISSN: 0015-5586 [Print] Poland
• PMID: 700505 (Publication Type: Journal Article)

Chemical References

• Iodine Radioisotopes
• L-Lactate Dehydrogenase
• Nucleotidases
• Glucuronidase

Topics

• Cell Line
• Chromatography, Gel
• Glucuronidase (metabolism)
• Iodine Radioisotopes
• L-Lactate Dehydrogenase (metabolism)
• Leukemia, Lymphoid
• Microsomes (enzymology)
• Nucleotidases (metabolism)
• Subcellular Fractions