Immunoassay of antigens and haptens by inhibition of passive immune hemolysis.

Abstract

Improved methods are presented for the detection of antigens and haptens by the use of the passive hemolysis inhibition test. The test is capable of detecting nanogram quantities of proteins (e.g., ferritin, IgE) and haptens (e.g., folinic acid, methotrexate). The method is also useful for studying quantitative and qualitative aspects of antibody-antigen interaction.

• Authors: T Borsos, V C Dunkel, J J Langone
• Journal: Journal of immunological methods (J Immunol Methods) Vol. 32 Issue 2 Pg. 105-14 ( 1980) ISSN: 0022-1759 [Print] Netherlands
• PMID: 6965695 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigen-Antibody Complex
• Antigens
• Haptens
• Leucovorin
• Methotrexate

Topics

• Animals
• Antibody Specificity
• Antigen-Antibody Complex (analysis)
• Antigens (analysis)
• Complement Fixation Tests (methods)
• Cross Reactions
• Dose-Response Relationship, Immunologic
• Erythrocytes (immunology)
• Haptens (analysis)
• Hemolysis
• Immunization
• Kinetics
• Leucovorin (blood, immunology)
• Methotrexate (immunology)
• Rabbits