HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The nude mouse. A possible experimental model for investigation of human thyroid tissue.

Abstract
The effect of transplanting hyperfunctioning human thyroid tissue to athymic, nude mice was explored. The thyroid tissue was obtained from a patient with diffuse, thyrotoxic goiter preoperatively treated with a beta-adrenoreceptor blocker. One transplant was placed in each groin of 4 nude mice. Light microscopy after 4 weeks showed that transplants consisted mainly of typical follicles; capillaries were common at the periphery of the transplant but not in between the follicles. The ultrastructure of the follicle cells in the transplants was similar to that of follicle cells in general. All 8 transplants accumulated 125I as revealed by external counting; the uptake in each transplant 24 h after administration of radioiodine was 4-25% of that in the thyroid of the transplanted mouse. The release of radioiodine, measured during a period of 14 days, from the thyroid of a transplanted mouse was delayed as compared to a nontransplanted control; this indicates that the mouse thyroid activity was suppressed due to hormone production in the transplants. Electron microscopic autoradiography 24 h after injection of 125I showed that protein-bound label in transplants was located mainly in follicle lumens. The radioiodine was incorporated in thyroglobulin as demonstrated by separation of soluble proteins from transplants by sucrose density gradient centrifugation. The present study shows that human thyroid tissue can be transplanted to nude mice with maintained structural and functional properties. Transplantation of human thyroid tissue might be a useful model for studies of thyroid diseases.
AuthorsS Smeds, B Anderberg, B Boeryd, L E Ericson, J Gillquist, J Persliden
JournalJournal of endocrinological investigation (J Endocrinol Invest) 1981 Jan-Mar Vol. 4 Issue 1 Pg. 11-5 ISSN: 0391-4097 [Print] Italy
PMID6894603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Thyroglobulin
  • Iodine
Topics
  • Animals
  • Disease Models, Animal
  • Graves Disease (metabolism, pathology)
  • Iodine (metabolism)
  • Male
  • Mice
  • Mice, Nude (physiology)
  • Microscopy, Electron
  • Thyroglobulin (metabolism)
  • Thyroid Gland (metabolism, transplantation, ultrastructure)
  • Transplantation, Heterologous

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: