[Commitment to erythroid differentiation and globin gene expression in the absence of heme accumulation in Friend leukemia cells].

Abstract

Friend leukemia cells (FLC) induced to erythroid differentiation by 5 mM hexamethylenebisacetamide (HMBA) are suitable for studies focused on the expression of different genes involved in the same differentiative program. In our studies FL cells have been cultured in alpha-medium (Gibco), 10% fetal calf serum (FCS, Flow Laboratories). Erythroid differentiation has been analysed by benzidine staining of the cells and globin synthesis by pulse-labelling the cells with 3H-leucine (New England Nuclear) and electrophoresis of newly-synthesized proteins on polyacrylamide gels. Hemoglobin production was determined by cellulose acetate gel electrophoresis. Our results demonstrate that heme synthesis is suppressed by the iron-chelator desferrioxamine. In addition "commitment" to erythroid differentiation and transcription and translation of globin mRNAs occur in HMBA-treated FLC in the absence of heme accumulation.

• Authors: R Gambari
• Journal: Bollettino della Societa italiana di biologia sperimentale (Boll Soc Ital Biol Sper) Vol. 59 Issue 5 Pg. 631-7 (May 30 1983) ISSN: 0037-8771 [Print] Italy
• Vernacular Title: "Commitment" al differenziamento eritroide ed espressione dei geni per le globine in assenza di accumulo di eme in cellule leucemiche di Friend.
• PMID: 6882562 (Publication Type: English Abstract, Journal Article)

Chemical References

• Acetamides
• Heme
• Globins
• hexamethylene bisacetamide

Topics

• Acetamides (pharmacology)
• Animals
• Cell Differentiation (drug effects)
• Electrophoresis, Cellulose Acetate
• Flow Cytometry
• Friend murine leukemia virus
• Gene Expression Regulation
• Globins (genetics)
• Heme (metabolism)
• Leukemia, Experimental (genetics, physiopathology)
• Mice