Inhibition of leukocyte adherence by potassium chloride extract of allogeneic squamous cell carcinoma of the human uterine cervix.

Abstract

Leukocyte adherence inhibition (LAI) assay was used to evaluate cell-mediated immunity in patients with invasive squamous cell carcinoma of the cervix. The reactivity of the peripheral blood leukocytes of these patients was evaluated after incubation with pooled extracts of allogeneic squamous cell carcinoma of the cervix. One hundred sixty-seven sets of LAI assays were performed on 54 individuals, including 23 patients with Stage I squamous cell carcinoma of the cervix, 9 patients with other stages of this tumor, 9 patients with unrelated tumors and 13 normal healthy volunteers. A protein concentration of one milligram per milliliter in the tumor extract and 10% fetal bovine serum in the feeding media gave the best results. Eighty-seven percent (28/32) of patients with squamous cell carcinoma of the cervix showed marked specific reactivity. No difference was found in the LAI indices of different stages of the disease.

• Authors: M M Dini, L Lenzi, I Faiferman
• Journal: American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction (Am J Reprod Immunol (1980)) Vol. 3 Issue 4 Pg. 188-90 (Jun 1983) ISSN: 0271-7352 [Print] United States
• PMID: 6881415 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens, Neoplasm
• Potassium Chloride

Topics

• Adult
• Aged
• Antigens, Neoplasm (immunology, isolation & purification)
• Carcinoma, Squamous Cell (immunology)
• Female
• Humans
• Immunity, Cellular
• Leukocyte Adherence Inhibition Test
• Middle Aged
• Neoplasms (immunology)
• Potassium Chloride
• Uterine Cervical Neoplasms (immunology)