Investigation of malignant hyperthermia: analysis of skeletal muscle proteins from normal and halothane sensitive pigs by two dimensional gel electrophoresis.

Abstract

Two dimensional gel analysis of skeletal muscles from normal pigs and from pigs which were homozygous for halothane sensitivity showed no obvious differences in the patterns of spots attributed to the major contractile proteins and glycolytic enzymes. In muscle from a sensitive pig which died of heat shock under anaesthesia there was a selective loss of glyceraldehyde-3-phosphate dehydrogenase and aldolase, presumably owing to proteolytic activity. The progressive loss of these enzymes under anaesthesia could contribute to the mechanism of heat production by diverting fructose 1,6 diphosphate into a futile cycle.

• Authors: P A Lorkin, H Lehmann
• Journal: Journal of medical genetics (J Med Genet) Vol. 20 Issue 1 Pg. 18-24 (Feb 1983) ISSN: 0022-2593 [Print] England
• PMID: 6842531 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Muscle Proteins
• Tropomyosin
• Troponin
• Glyceraldehyde-3-Phosphate Dehydrogenases
• Myosins
• Fructose-Bisphosphate Aldolase
• Halothane

Topics

• Actins (analysis)
• Animals
• Electrophoresis, Polyacrylamide Gel
• Fructose-Bisphosphate Aldolase (analysis)
• Glyceraldehyde-3-Phosphate Dehydrogenases (analysis)
• Halothane
• Homozygote
• Isoelectric Focusing
• Malignant Hyperthermia (genetics, metabolism)
• Muscle Proteins (analysis)
• Myosins (analysis)
• Swine
• Tropomyosin (analysis)
• Troponin (analysis)