In vitro formation of different tubulin polymers from purified tubulin of Ehrlich ascites tumor cells.

Abstract

Preparations of cycled tubulin from Ehrlich ascites tumor cells contain several accessory proteins; once or twice cycled microtubule preparations are usually composed of fibers 10 nm in diameter, but lack vimentin. Highly purified tubulin consists of alpha- and beta-tubulin and a minor component which was identified by peptide mapping as a second beta-chain. This pure tubulin is able to form in vitro at low concentrations (1 mg protein/ml) fibers of about 10 nm width, and at higher concentrations (3.5 mg protein/ml) normal microtubules.

• Authors: K H Doenges, H P Zimmermann, M Flemming, D Schroeter
• Journal: FEBS letters (FEBS Lett) Vol. 151 Issue 2 Pg. 286-90 (Jan 24 1983) ISSN: 0014-5793 [Print] England
• PMID: 6832357 (Publication Type: Journal Article)

Chemical References

• Macromolecular Substances
• Tubulin

Topics

• Animals
• Carcinoma, Ehrlich Tumor (ultrastructure)
• Macromolecular Substances
• Mice
• Microscopy, Electron
• Microtubules (ultrastructure)
• Molecular Weight
• Tubulin (isolation & purification, metabolism)