Abstract |
Native (n) but not single stranded (ss) DNA was found to induce release of 3H-serotonin (5-HT) from platelets of the majority of normal individuals. However, ssDNA markedly enhanced 5-HT release induced by heat-aggregated IgG (aggIgG), while less enhancement was seen using nDNA. Similar enhancement was produced by polyinosinic acid but not by polyinosinic:polycytidylic acid. The ability of ssDNA to potentiate aggIgG-induced 5-HT release seemed specific for aggIgG, since no effect on ADP or epinephrine-induced release was observed and thrombin-induced release was inhibited. In contrast, nDNA in high concentrations (100 micrograms/ml) potentiated ADP, epinephrine, and thrombin-induced 5-HT release. These results suggest that ss-and nDNA may interact with platelets by different mechanisms and provide a means by which DNA, released at sites of tissue injury, could modulate the role of platelets in the inflammatory response. The ability of DNA to enhance the aggIgG-induced platelet release reaction may be important in immune complex diseases such as systemic lupus erythematosus.
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Authors | C A Dorsch, J Killmayer |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 26
Issue 2
Pg. 179-85
(Feb 1983)
ISSN: 0004-3591 [Print] United States |
PMID | 6824515
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Single-Stranded
- Immunoglobulin G
- Poly I
- Serotonin
- Adenosine Diphosphate
- DNA
- Thrombin
- Poly I-C
- Imipramine
- Epinephrine
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Topics |
- Adenosine Diphosphate
(pharmacology)
- Blood Platelets
(metabolism)
- DNA
(pharmacology)
- DNA, Single-Stranded
(pharmacology)
- Epinephrine
(pharmacology)
- Humans
- Imipramine
(pharmacology)
- Immunoglobulin G
(physiology)
- Poly I
(pharmacology)
- Poly I-C
(pharmacology)
- Serotonin
(metabolism)
- Thrombin
(pharmacology)
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