Increase of anti-ulcerogenic activity by reduction and alkylation of disulfide bonds of a globulin fraction from bovine serum.

Abstract

Bovine serum was subjected to ammonium sulfate fractionation, and subsequently chromatographed on DEAE-cellulose column to obtain a globulin protein having a weak anti-ulcerogenic activity, Fr.II-A. Agar immunoelectrophoresis and immunodiffusion using rabbit antiserum against bovine IgG revealed that Fr.II-A from bovine serum shared antigenic determinant with bovine IgG. Furthermore, Fr.II-A showed a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. By reducing and alkylating the disulfide bonds by 2-mercaptoethanol and iodoacetic acid, Fr.II-A was separated into two subfragments in the same manner as in the case of immunoglobulin to produce Fr.L (molecular weight; 24000) and Fr.H (molecular weight; 50000). Fr.L showed anti-ulcerogenic activity up to about ten times greater than that of original Fr.II-A, but in the case of Fr.H, the activity increased approximately several times. Fr.L showed significant activity in preventing ulcer formation and gastric juice secretion in pylorus-ligated rats, and was also effective in reducing index of the phenylbutazone-induced ulcer.

• Authors: T Mimura, K Kameda, T Terada, I Kohda, C Tanaka, S Aonuma
• Journal: Journal of pharmacobio-dynamics (J Pharmacobiodyn) Vol. 5 Issue 5 Pg. 301-7 (May 1982) ISSN: 0386-846X [Print] Japan
• PMID: 6811723 (Publication Type: Journal Article)

Chemical References

• Anti-Ulcer Agents
• Disulfides
• Globulins

Topics

• Alkylation
• Animals
• Anti-Ulcer Agents
• Chromatography, Gel
• Disulfides
• Electrophoresis, Polyacrylamide Gel
• Globulins (pharmacology)
• Immunochemistry
• Immunodiffusion
• Male
• Oxidation-Reduction
• Rats
• Rats, Inbred Strains
• Stomach Ulcer (chemically induced, drug therapy)