Mechanism for pulmonary protection by lipopolysaccharide pseudomonas vaccine.

Abstract

Recent studies in guinea pigs indicated that active vaccination with a cell wall-derived, lipopolysaccharide (LPS) pseudomonas vaccine confers specific protection against acute pneumonia. This study analyzed the immune mechanisms by which LPS pseudomonas vaccine offers local protection to the lung. Neither parenteral vaccination nor direct exposure of lung tissues to living Pseudomonas activated alveolar macrophages. However, serum opsonic activity that specifically enhanced phagocytosis of Pseudomonas by alveolar macrophages was significantly increased (P < 0.05) in vaccinated animals. Serum pseudomonas opsonins were heat-stable but were sensitive to reduction of macroglobulins by 2-mercaptoethanol. Within 3 hr after establishment of experimental pseudomonas pneumonia, a fourfold increase in local pseudomonas opsonins was found in bronchial fluids from vaccinated animals, presumably secondary to diffusion of serum proteins into local inflammatory fluids. Thus, Pseudomonas-specific opsonic antibody appears necessary to augment alveolar macrophage phagocytosis after LPS vaccination, and local vaccination of the respiratory tract is not required to provide adequate local pseudomonas opsonins during acute pneumonia.

• Authors: J E Pennington, D Kuchmy
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 142 Issue 2 Pg. 191-8 (Aug 1980) ISSN: 0022-1899 [Print] United States
• PMID: 6774032 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Vaccines
• Lipopolysaccharides
• Opsonin Proteins

Topics

• Animals
• Antibodies, Bacterial (biosynthesis)
• Antibody Specificity
• Antigens, Bacterial
• Bacterial Vaccines (therapeutic use)
• Guinea Pigs
• Lipopolysaccharides (therapeutic use)
• Macrophages (immunology)
• Opsonin Proteins (immunology)
• Pneumonia (prevention & control)
• Pseudomonas aeruginosa (immunology)