[Acute hepatic insufficiency in guinea pigs. Experimental model caused by the injection of monoethanolamine oleate].

Abstract

An appropriate animal model of acute fulminant hepatic failure was developed in the guinea pig by he intrabiliary administration of monoethanolamine oleate. The animals were assigned in two experimental groups: 1) ethanolamine group - 42 guinea pigs that received intrabiliary 2,5 ml injection of monoethanolamine oleate; 2) control group - 18 guinea pigs subjected to intrabiliary 2,5 ml administration of saline. The intrabiliary administration of the ethanolamine oleate resulted in massive liver injury with 85,9% of hepatic coma during the first 96 hr. The liver damage was characterized by clinical manifestations (anorexia, increasing stupor, muscle wasting and deep coma), serum biochemical tests (elevations of serum transaminases, bilirubins , alkaline phosphatase), studies of blood coagulation (prothrombin and partial thromboplastin times were markedly prolonged and the concentration of fibrinogen decreased) and histopathological findings (massive hepatic necrosis). This animal model appears promising for future studies of the pathogenesis and treatment of acute hepatic failure.

• Authors: A R de Macedo, C E Bacchi, C T Spadella, M R Montenegro, M R de Almeida, W S Hossne
• Journal: Arquivos de gastroenterologia (Arq Gastroenterol) 1983 Jul-Sep Vol. 20 Issue 3 Pg. 92-102 ISSN: 0004-2803 [Print] Brazil
• Vernacular Title: Insuficiência hepática aguda no cobaio. Modelo experimental causado pela injecão de oleato de monoetanolamina.
• PMID: 6677256 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oleic Acids
• ethanolamine oleate

Topics

• Animals
• Chemical and Drug Induced Liver Injury
• Disease Models, Animal
• Guinea Pigs
• Hepatic Encephalopathy (chemically induced)
• Liver (pathology, physiopathology)
• Liver Diseases (pathology, physiopathology)
• Oleic Acids (toxicity)