Sialic acid metabolism in rat liver: effect of carbon tetrachloride.

Abstract

Sialic acid metabolism was investigated in the livers of control rats and of rats treated with a single oral dose (1.5 ml/kg body weight) of carbon tetrachloride. The main change observed during the necrotic stage of CCl4 poisoning (18 h after treatment) was a highly significant reduction in sialyltransferase activity. Slight reciprocal changes in neuraminidase activities, i.e., a small decrease in cytosolic neuraminidase and a small increase in the membrane bound enzyme were also observed. At 72 h after CCl4 treatment, during the stage of liver regeneration, the main change was a marked elevation in membrane-bound neuraminidase (two fold above control values). Moderate increases in the specific activities of CMP-N-acetylneuraminic acid synthetase and sialyltransferase were also observed. A considerable decrease in the sialic acid content of the isolated smooth endoplasmic reticulum (one half of control values) was detected at 72 h after CCl4 administration. The sialic acid content of the rough endoplasmic reticulum, on the other hand, remained at control levels.

• Authors: G S Kishore, R E Nordquist, R Carubelli
• Journal: Life sciences (Life Sci) Vol. 33 Issue 21 Pg. 2129-36 (Nov 21 1983) ISSN: 0024-3205 [Print] Netherlands
• PMID: 6645793 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Sialic Acids
• Sialyltransferases
• Neuraminidase

Topics

• Animals
• Carbon Tetrachloride Poisoning (metabolism, pathology)
• Kinetics
• Liver (metabolism, ultrastructure)
• Liver Regeneration
• Male
• Microscopy, Electron
• Necrosis
• Neuraminidase (metabolism)
• Rats
• Rats, Inbred Strains
• Sialic Acids (metabolism)
• Sialyltransferases (metabolism)