Quantitation of platelet membrane glycoproteins in Glanzmann's thrombasthenia and the Bernard-Soulier syndrome by electroimmunoassay.

Abstract

A rapid and precise electroimmunoassay for quantitation of glycoprotein Ib and IIb-IIIa in platelet lysates is described. Using this method, platelets from two patients with Type II thrombasthenia contained 8% and 12% of normal levels of IIb-IIIa, whereas IIb-IIIa was undetectable in platelets of one Type I thrombasthenic patient. The level of Ib in two patients with Bernard-Soulier syndrome was essentially nil. The increased precision of this assay, when compared to CIE, allows for estimations of glycoproteins levels varying by no more than a few percent.

• Authors: S M Kristopeit, T J Kunicki
• Journal: Thrombosis research (Thromb Res) Vol. 36 Issue 2 Pg. 133-42 (Oct 15 1984) ISSN: 0049-3848 [Print] United States
• PMID: 6506033 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Blood Coagulation Factors
• Glycoproteins
• Membrane Proteins

Topics

• Antibodies, Monoclonal
• Blood Coagulation Factors (analysis)
• Blood Platelets (analysis)
• Electrophoresis, Agar Gel
• Glycoproteins (blood)
• Humans
• Immunoelectrophoresis
• Membrane Proteins (blood)
• Thrombocytopenia (blood)