A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations.

Abstract	This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of "in situ" complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described.
Authors	A Villanueva, J C Stockert, R Armas-Portela
Journal	Histochemistry (Histochemistry) Vol. 81 Issue 1 Pg. 103-4 ( 1984) ISSN: 0301-5564 [Print] Germany
PMID	6469718 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Fluorescent Dyes Nucleic Acids
Topics	Animals Carcinoma, Ehrlich Tumor (analysis) Chickens Fluorescent Dyes Histocytochemistry Microscopy, Fluorescence Nucleic Acids (analysis) Spectrometry, Fluorescence

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