[Detection of the antigens of the virus of Congo-Crimean hemorrhagic fever by solid-phase immunoenzyme analysis and by the indirect hemagglutination reaction].

Abstract

From a batch of immunoglobulin to Crimean-Congo hemorrhagic fever virus, an immunosorbent and a conjugate with peroxidase (RZ-2.7) for solid-phase enzyme immunoassay (EIA) as well as erythrocyte diagnostic preparation for indirect hemagglutination test (IHA) were obtained. Direct EIA and IHA were used to study the time course of accumulation of virus antigen in brain tissue of newborn white mice. Antigen titres by EIA were 4-8 times as low as by IHA. No functional relationship was found between antibody titres and those of infectious virus.

• Authors: V A Tsar'kova, V P Nikolaev
• Journal: Voprosy virusologii (Vopr Virusol) 1984 Nov-Dec Vol. 29 Issue 6 Pg. 724-6 ISSN: 0507-4088 [Print] Russia (Federation)
• Vernacular Title: Vyiavlenie antigenov virusa Kongo-Krymskoĭ gemorragicheskoĭ likhoradki s pomoshch'iu tverdofaznogo immunofermentnogo analiza i reaktsii nepriamoĭ gemaggluitinatsii.
• PMID: 6442044 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antigens, Viral

Topics

• Animals
• Animals, Newborn
• Antigens, Viral (analysis)
• Bunyaviridae (immunology)
• Evaluation Studies as Topic
• Hemagglutination Tests
• Hemorrhagic Fever Virus, Crimean-Congo (immunology)
• Immunoenzyme Techniques
• Mice