Abstract |
The effect of 2-chloroadenosine on bone resorption was studied in calvarial bones from 6-7-day-old mice in organ culture. 2-Chloroadenosine stimulated the mobilization of minerals (40Ca, 45Ca) and increased the degradation of matrix ([3H] proline) from the bones. The nucleoside also caused an increased release of beta-glucuronidase, a lysosomal enzyme. In doses above 30 microM 2-chloroadenosine was cytotoxic, as evidenced by an increased release of lactate dehydrogenase. 2-Chloroadenosine-stimulated resorption could be inhibited by calcitonin, increased concentration of phosphate in culture medium, cortisone, dexamethasone, indomethacin, naproxen, meclofenamic acid and 5,8,11,14-eicosatetraynoic acid. 2-Chloroadenosine was much more sensitive to inhibition by dexamethasone than was parathyroid hormone. The response to the maximal dose of 2-chloroadenosine could not be enhanced by parathyroid hormone, 1 alpha-hydroxyvitamin D-3 and prostaglandin E2. An exposure to 2-chloroadenosine for 12 h was not sufficient to produce prolonged resorption. The results suggest that 2-chloroadenosine stimulated bone resorption by a process which is dependent on osteoclastic activity. The possibility that the effect of 2-chloroadenosine, either directly or indirectly, is related to formation of prostaglandins is discussed in the light of the above data.
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Authors | U Lerner, B B Fredholm |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 757
Issue 2
Pg. 226-34
(May 25 1983)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 6405798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Arachidonic Acids
- Parathyroid Hormone
- Prostaglandins E
- 2-Chloroadenosine
- Arachidonic Acid
- Glucuronidase
- Calcitriol
- Adenosine
- Dinoprostone
- Calcium
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Topics |
- 2-Chloroadenosine
- Adenosine
(analogs & derivatives, pharmacology)
- Animals
- Arachidonic Acid
- Arachidonic Acids
(metabolism)
- Bone Resorption
(drug effects)
- Bone and Bones
(metabolism)
- Calcitriol
(pharmacology)
- Calcium
(metabolism)
- Cell Survival
(drug effects)
- Culture Techniques
- Dinoprostone
- Glucuronidase
(metabolism)
- Mice
- Parathyroid Hormone
(pharmacology)
- Prostaglandins E
(pharmacology)
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