Abstract |
An enzyme-linked immunosorbent assay (ELISA) was used with a purified alpha-toxin preparation to measure the serum IgG, IgM and IgA response in staphylococcal septicaemia and endocarditis. ELISA for IgG antibodies against alpha-toxin was found to be more sensitive than the neutralization test (ASTA). IgM and IgA antibody determination was found to be of limited diagnostic value. A correlation between IgG antibodies to alpha-toxin and purified beta-toxin was found in ELISA, although antibody determination to beta-toxin was a less sensitive diagnostic method. The highest diagnostic sensitivity in deep staphylococcal infections was obtained by parallel performance of ELISA to alpha-toxin and purified teichoic acid. By this approach, 32/35 (91%) patients with endocarditis, 12/14 (86%) with complicated septicaemia and 15/22 (68%) with uncomplicated septicaemia showed increased titres in samples drawn between days 7-30 of disease. Diagnostic sensitivity was further increased to 31/32 (97%) positive patients, when paired or multiple samples from patients with septicaemic staphylococcal disease were analysed.
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Authors | M Granström, I Julander, R Möllby |
Journal | Scandinavian journal of infectious diseases. Supplementum
(Scand J Infect Dis Suppl)
Vol. 41
Pg. 132-9
( 1983)
ISSN: 0300-8878 [Print] England |
PMID | 6379858
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Bacterial
- Bacterial Toxins
- Hemolysin Proteins
- Teichoic Acids
- staphylococcal alpha-toxin
- Sphingomyelin Phosphodiesterase
- hlb protein, Staphylococcus aureus
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Topics |
- Antibodies, Bacterial
(analysis)
- Bacterial Toxins
(immunology)
- Endocarditis, Bacterial
(diagnosis, immunology)
- Enzyme-Linked Immunosorbent Assay
- Furunculosis
(diagnosis, immunology)
- Hemolysin Proteins
(immunology)
- Humans
- Immunoenzyme Techniques
- Osteomyelitis
(diagnosis, immunology)
- Sepsis
(diagnosis, immunology)
- Sphingomyelin Phosphodiesterase
- Staphylococcal Infections
(diagnosis, immunology)
- Staphylococcus aureus
(immunology)
- Teichoic Acids
(immunology)
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