The neurotoxins 2,5-hexanedione and acrylamide promote aggregation of intermediate filaments in cultured fibroblasts.

Axonal swellings associated with large aggregates of neurofilaments are characteristic of neuropathies caused by chemical neurotoxins (n-hexane, methyl n-butyl ketone, and acrylamide) or giant axonal neuropathy (GAN--an autosomal recessive genetic disease). In GAN, filamentous aggregates have been shown also to occur in other cell types including cultured skin fibroblasts. Therefore, we studied the effects of 2,5-hexanedione (the neurotoxic metabolite of n-hexane and methyl n-butyl ketone) and acrylamide on normal human skin fibroblasts in tissue culture. We show that both neurotoxins induce aggregation of intermediate filaments of the vimentin type in the cultured fibroblasts without disrupting microtubules.
AuthorsH D Durham, S D Pena, S Carpenter
JournalMuscle & nerve (Muscle Nerve) 1983 Nov-Dec Vol. 6 Issue 9 Pg. 631-7 ISSN: 0148-639X [Print] United States
PMID6361548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Acrylamides
  • Hexanones
  • Intermediate Filament Proteins
  • Ketones
  • Tubulin
  • Vimentin
  • Acrylamide
  • 2,5-hexanedione
  • Acrylamide
  • Acrylamides (pharmacology)
  • Cells, Cultured
  • Child
  • Cytoskeleton (drug effects)
  • Fibroblasts (drug effects)
  • Fluorescent Antibody Technique
  • Hexanones (pharmacology)
  • Humans
  • Intermediate Filament Proteins (metabolism)
  • Ketones (pharmacology)
  • Male
  • Microscopy, Electron
  • Skin
  • Tubulin (metabolism)
  • Vimentin

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!

Choose Username:
Verify Password:
Enter Code Shown: