[Transport of Rb+ via activated sodium channels of clone N 18 phi 1 neuroblastoma cells].

Abstract

A study was made of the Rb+ transport via activated sodium channels of clone N 18 phi 1 neuroblastoma cells cultured in the Eagle medium with 10% bovine serum. The time of population doubling was about 10 h. The cell differentiation was induced by adding bromdeoxyuridine in a concentration of 1-4 10(-5) M. The cells contained 172 +/- 12 and 340 +/- 35 micrograms of protein per 10(6) cells at the logarithmic growth phase and in differentiated state, respectively. It is shown that veratrin produced a 1.3-fold increase in the rate of 86Rb+ removal from undifferentiated cells and 2.5-fold increase in that from differentiated cells. Tetrodotoxin removed completely the effect of veratrin. A conclusion is made on the presence of a new clone of fast sodium channels in cell membranes.

• Authors: N M Gulaia, V K Lishko, G L Volkov, N N Govseeva
• Journal: Ukrainskii biokhimicheskii zhurnal (1978) (Ukr Biokhim Zh (1978)) 1983 Nov-Dec Vol. 55 Issue 6 Pg. 657-61 ISSN: 0201-8470 [Print] Ukraine
• Vernacular Title: Transport Rb+ cherez aktivirovannye natrievye kanaly kletok neĭroblastomy klona N 18 fi 1.
• PMID: 6318414 (Publication Type: English Abstract, Journal Article)

Chemical References

• Ion Channels
• Tetrodotoxin
• Sodium
• Rubidium

Topics

• Animals
• Biological Transport, Active (drug effects)
• Cell Differentiation
• Cell Line
• Ion Channels (drug effects, metabolism)
• Kinetics
• Mice
• Neuroblastoma (metabolism)
• Rubidium (metabolism)
• Sodium (metabolism)
• Tetrodotoxin (pharmacology)