Activation of polycyclic hydrocarbons in Reuber H4-II-E hepatoma cells. An in vitro system for the induction of SCEs.

Abstract

Activation of polycyclic aromatic hydrocarbons (PAHs) was examined in the Reuber H4-II-E established cell line without the use of exogenous enzyme preparations. Metabolism of PAHs to genotoxic products was determined by the induction of sister-chromatid exchanges (SCEs). The induction of SCEs followed a dose-response pattern with plateaus at high doses of PAH. The effects of metabolic enzyme inducers (3-methylcholanthrene, phenobarbital, Aroclor 1254) and the epoxide hydrase inhibitor 1,1,1-trichloropropylene oxide were assessed as changes in SCE induction and enhanced production of water-soluble metabolites. Results indicate that Reuber H4-II-E cells can be employed in the testing of carcinogens activated by the P1-450 monooxygenase system and would be a useful in vitro system for the study of mechanisms of metabolic induction and their effect on genetic toxicity.

• Authors: R G Dean, G Bynum, D Jacobson-Kram, E Hadley
• Journal: Mutation research (Mutat Res) Vol. 111 Issue 3 Pg. 419-27 (Nov 1983) ISSN: 0027-5107 [Print] Netherlands
• PMID: 6316133 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Benzopyrenes
• Mutagens
• Polycyclic Compounds
• Benzo(a)pyrene
• Methylcholanthrene
• 9,10-Dimethyl-1,2-benzanthracene

Topics

• 9,10-Dimethyl-1,2-benzanthracene (toxicity)
• Animals
• Benzo(a)pyrene
• Benzopyrenes (toxicity)
• Crossing Over, Genetic (drug effects)
• In Vitro Techniques
• Kinetics
• Liver Neoplasms, Experimental (physiopathology)
• Methylcholanthrene (toxicity)
• Mutagens (toxicity)
• Polycyclic Compounds (toxicity)
• Sister Chromatid Exchange (drug effects)
• Structure-Activity Relationship