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The calcitonin receptor on T 47D breast cancer cells. Evidence for glycosylation.

Abstract
The glycosyl nature of the receptor for the peptide hormone calcitonin has been investigated in a human breast cancer cell line, T 47D. Studies have been carried out to assess the ability of various lectins and of the antibiotic tunicamycin to inhibit specific binding of calcitonin to the cells, to reduce cross-linking of photoactive calcitonin to a macromolecular receptor component and to influence calcitonin stimulation of cyclic AMP. Pre-incubation of cells with low concentrations of tunicamycin for 72 h resulted in a reduction of total specific binding by approx. 80% and a 40% reduction in calcitonin-stimulated adenylate cyclase; formation of the cross-linked receptor component was also inhibited. Wheat-germ lectin showed the most marked inhibition of total specific binding and cyclic AMP production. However, cross-linking of photoactive calcitonin to receptor component was totally inhibited by this lectin. Soya-bean lectin brought about very little reduction in total specific binding but had more profound effects on calcitonin-stimulated cyclic AMP production and cross-linking of photoactive calcitonin. Concanavalin A and lentil lectin showed some inhibition of all parameters. The data indicate that the calcitonin receptor in T 47D cells is associated with glycosyl moieties, the major contributors of which are N-acetyl-D-glucosamine residues, but N-acetyl-D-galactosamine and mannose residues are also associated.
AuthorsJ M Moseley, D M Findlay, J J Gorman, V P Michelangeli, T J Martin
JournalThe Biochemical journal (Biochem J) Vol. 212 Issue 3 Pg. 609-16 (Jun 15 1983) ISSN: 0264-6021 [Print] England
PMID6309149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Lectins
  • Macromolecular Substances
  • Receptors, Calcitonin
  • Receptors, Cell Surface
  • Tunicamycin
  • Calcitonin
  • Cyclic AMP
  • Adenylyl Cyclases
Topics
  • Adenylyl Cyclases (metabolism)
  • Breast Neoplasms (metabolism)
  • Calcitonin (metabolism)
  • Cell Line
  • Cyclic AMP (metabolism)
  • Enzyme Activation (drug effects)
  • Female
  • Humans
  • Lectins (pharmacology)
  • Macromolecular Substances
  • Protein Biosynthesis
  • Receptors, Calcitonin
  • Receptors, Cell Surface (drug effects, metabolism)
  • Tunicamycin (pharmacology)

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