Lymphocyte cytotoxicity in x-irradiation-induced rat small bowel adenocarcinoma. III. Blocking by 3 M KCL extract.

Abstract

Hypertonic salt extracts (3 M KCl) of x-irradiation-induced Holtzman rat small bowel adenocarcinomas blocked the in vitro destruction of allogeneic cultured cells of this malignancy by sensitized lymphoid cells obtained from tumor-bearing animals. The protective effect were mediated by a blocking action at both the effector and the target cell level. The extracts were separated into 50% ammonium sulfate soluble and insoluble fractions with the soluble fraction being more effective in blocking the cytotoxic responses through interaction with the lymphoid cells whereas the insoluble one had a greater effect upon tumor target cells. Associated with both fractions was the oncofetal glycoprotein previously identified with the cellular membrane of this x-ray-induced malignancy. Immunoglobulins were identified with insoluble fraction; some were able to bind the oncofetal protein, thus clasifying it as a fetal antigen. The protective effects of the soluble fraction and this neoantigen were found to be citric acid labile, whereas the effects due to the insoluble fraction were unchanged.

• Authors: R H Stevens, G P Brooks, J W Osborne, K L Hoffman, A J Lawson
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 120 Issue 1 Pg. 335-9 (Jan 1978) ISSN: 0022-1767 [Print] United States
• PMID: 627724 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Potassium Chloride
• Ammonium Sulfate

Topics

• Adenocarcinoma (immunology)
• Ammonium Sulfate
• Animals
• Antigens, Neoplasm
• Cytotoxicity, Immunologic (drug effects)
• Fetus (immunology)
• Intestinal Neoplasms (immunology)
• Lymphocytes (drug effects, immunology)
• Male
• Neoplasms, Experimental (immunology)
• Neoplasms, Radiation-Induced (immunology)
• Potassium Chloride (pharmacology)
• Rats