Deoxyribonuclease I sensitivity of plasmid genomes in teratocarcinoma-derived stem and differentiated cells.

Abstract

The DNase I (EC 3.1.21.1) sensitivities of the simian virus 40 (SV40) genome, the pBR322 genome, and the herpes simplex virus type 1 thymidine kinase (HSV-1 tk) gene have been compared in teratocarcinoma-derived stem (12-1) and differentiated (12-1a) cell lines established by transfection of thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)-deficient F9 cells with DNA from a tripartite plasmid genome consisting of the pBR322 genome, the SV40 genome, and the HSV-1 tk gene. HSV-1 tk is present in both stem and differentiated cells; SV40 early proteins are present in differentiated cells but not in stem cells; the pBR322 genome is not expressed in either cell type. The SV40 and pBR322 genomes are more sensitive to DNase I digestion in stem cells than in differentiated cells, reflecting the DNase I-hypersensitivity of total stem-cell chromatin. The HSV-1 tk gene is the least sensitive to DNase I digestion in both cell types.

• Authors: K Huebner, A Linnenbach, S Weidner, G Glenn, C M Croce
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 78 Issue 8 Pg. 5071-5 (Aug 1981) ISSN: 0027-8424 [Print] United States
• PMID: 6272288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Chromatin
• DNA, Bacterial
• Thymidine Kinase
• Deoxyribonucleases

Topics

• Animals
• Cell Differentiation
• Cells, Cultured
• Chromatin (ultrastructure)
• DNA, Bacterial (metabolism)
• Deoxyribonucleases (metabolism)
• Gene Expression Regulation
• Genes, Viral
• Mice
• Plasmids
• Simian virus 40 (genetics)
• Simplexvirus (enzymology)
• Teratoma (physiopathology)
• Thymidine Kinase (genetics)