Effect of Corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in BALB/c mice.

Abstract

Treatment of 4- to 6-week-old, 18- to 22-g male BALB/c mice with 0.6 mg of Corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (HSV-2). Optimal protection occurred when C. granulosum was injected 1 week before HSV-2 infection. C. granulosum-induced resistance to HSV-2 lasted up to 4.5 weeks. Studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated immunity in HSV-2 infection. However, C. granulosum protection was not transferable with spleen cells from C. granulosum-treated animals nor was C. granulosum treatment capable of completely overcoming the increased mortality which resulted from antilymphocyte serum treatment of HSV-2 infected mice. Under our experimental conditions, silica did not affect the mortality in BALB/c mice due to HSV-2 but significantly reduced the protective effects induced by C. granulosum. Attempts to transfer protection of HSV-2 challenge in suckling mice by using either glass-adherent or mixed peritoneal cells from either HSV-2-immunized or C. granulosum-treated animals were unsuccessful.

• Authors: D A Gabrielson, J J Kelleher, J Varani
• Journal: Infection and immunity (Infect Immun) Vol. 30 Issue 3 Pg. 791-6 (Dec 1980) ISSN: 0019-9567 [Print] United States
• PMID: 6262241 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bacterial Vaccines
• Immune Sera
• Silicon Dioxide

Topics

• Animals
• Animals, Newborn
• Bacterial Vaccines (administration & dosage, immunology)
• Corynebacterium (immunology)
• Immune Sera
• Immunity, Cellular
• Immunization
• Male
• Mice
• Mice, Inbred BALB C
• Silicon Dioxide (immunology)
• Simplexvirus (pathogenicity)
• Spleen (immunology, transplantation)