Inactivation of the scrapie agent by pronase.

Abstract

Scrapie agent derived from infected mouse brain was partially purified by agarose-polyacrylamide gel electrophoresis. The preparation was subjected to treatment with RNase A, DNase I, pronase, sodium dodecyl sulfate and sodium dodecyl sulfate-pronase combination. Almost total inactivation of scrapie infectivity resulted when the partially purified scrapie agent was treated with sodium dodecyl sulfate-pronase combination whereas the treatment with sodium dodecyl sulfate or pronase alone reduced the infectivity by about 98%. No significant reduction of scrapie infectivity was observed with either RNase A or DNase I treatments. These results confirm that the transmission of scrapie cannot be achieved in the absence of a protein component.

• Authors: H J Cho
• Journal: Canadian journal of comparative medicine : Revue canadienne de medecine comparee (Can J Comp Med) Vol. 47 Issue 4 Pg. 494-6 (Oct 1983) ISSN: 0008-4050 [Print] Canada
• PMID: 6230145 (Publication Type: Journal Article)

Chemical References

• Prions
• Sodium Dodecyl Sulfate
• Endodeoxyribonucleases
• Deoxyribonuclease I
• Ribonuclease, Pancreatic
• Pronase

Topics

• Animals
• Deoxyribonuclease I
• Electrophoresis, Agar Gel
• Electrophoresis, Polyacrylamide Gel
• Endodeoxyribonucleases (pharmacology)
• Mice
• Prions (drug effects, isolation & purification)
• Pronase (pharmacology)
• Ribonuclease, Pancreatic (pharmacology)
• Sodium Dodecyl Sulfate (pharmacology)