Abstract |
An antigenic determinant present in the cytoplasm, but not on the surface membrane of human Ia+ cells, is defined by a monoclonal antibody (VIC-Y1) and is shown by immunoprecipitation and by NEPHGE to be expressed by Ia oligomers. Immunoprecipitations of cellfree translates and of purified Ia subunits indicate that the VIC-Y1 determinant is located on the Ia gamma-( invariant) chain, as well as on two other related molecules, provisionally termed gamma 2 and gamma 3. Within our experimental conditions, the three forms of gamma-chains co-precipitate exclusively with Ia oligomers. As detected by VIC-Y1 and in the limits of our assays, gamma-chains could not be found at the cell surface; their tissue distribution, determined by cytoplasmic indirect immunofluorescence with VIC-Y1, closely resembles that of Ia antigens, with the possible exception of acute lymphatic leukemia cells (Ia+, gamma-chain-).
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Authors | V Quaranta, O Majdic, G Stingl, K Liszka, H Honigsmann, W Knapp |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 132
Issue 4
Pg. 1900-5
(Apr 1984)
ISSN: 0022-1767 [Print] United States |
PMID | 6199417
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Epitopes
- Histocompatibility Antigens Class II
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- B-Lymphocytes
(immunology)
- Cytoplasm
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Epitopes
(genetics, immunology)
- Fluorescent Antibody Technique
- Genes, MHC Class II
- Histocompatibility Antigens Class II
(analysis, immunology, isolation & purification)
- Humans
- Mice
- Mice, Inbred BALB C
- Polymorphism, Genetic
- Rabbits
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