Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods.

Abstract

A comparison of five immunohistochemical methods for the demonstration of keratins in human ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase--antiperoxidase (PAP), indirect peroxidase, or the avidin--biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

• Authors: R B Nagle, V A Clark, K M McDaniel, J R Davis
• Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 31 Issue 8 Pg. 1010-4 (Aug 1983) ISSN: 0022-1554 [Print] United States
• PMID: 6190854 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Formaldehyde
• Keratins

Topics

• Female
• Fluorescent Antibody Technique
• Formaldehyde
• Freezing
• Histocytochemistry (methods)
• Humans
• Immunoenzyme Techniques
• Keratins (analysis)
• Ovarian Neoplasms (analysis, pathology)